rubycon_YXF
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电容品牌大全(转)电容品牌大全主板厂商惯用电容品牌富士康Rubycon Sanyo华擎KZG KZE升技Rubycon技嘉Rubycon KZG OST磐正Sanyo OST GSC微星KZG OST华硕Nichicon KZG硕泰克Sanyo Sacon捷波GSC七彩虹Taicon按照Intel主板技术白皮书的介绍,主板CPU插槽附近的滤波电容单个容量最低要求为1000μF。
大部分主板上常见电容的容量为2200μF,好的主板采用3500μF甚至更高容量的电容。
而在Intel的原装主板上,一般单个电容容量都在3300μF以上,这就是Intel主板极其稳定的重要原因之一。
可见“电容决定主板质量”这话一点不假。
下面是主要电容品牌的体系图,都是从电容厂商的网站上DOWN下来的,买电容的时候可以参考一下:目前只找到SANYO、nichicon的体系图,其它厂商只提供PDF文档,有兴趣的可以去看:SANYO: www.secc.co.jp/english/index.htmlnichicon: chemicon: rubycon: teapo: OST: 总结一下,比较适合主板使用的电容必须是\"Low Impedance & ESR\"、\"Very Low Impedance & ESR\"、\"Ultra Low Impedance & ESR\" 且温度为105C的,Ultra的最好SANYO:MV-WG、MV-WX、MV-SWG、MB-UWG、MB-EXR等系列nichicon: H开头的系列,P开头的系列chemicon: KZE、KZG系列rubycon: YXF、YXG、ZL、ZLH、MBZ、MCZ系列teapo: SC、SM、SZ系列OST: RLP、RLZ系列系列太多了,列举不完。
在可选的系列中,再根据PDF的资料,选择寿命比较长的就好了,一般PC 只需在2000小时以上就差不多了,5000小时以上的一是难买,二是贵。
单相电子式(不分时)电能表技术条件收藏此信息打印该信息添加:用户发布来源:未知1.适用X围本技术条件适用于新生产的,在XX省电网运行中的单相用户的液晶显示不分时电能表。
本技术条件是指导各局订货和验收的规X。
本技术条件中未明确之处,应满足现行有效的国家及行业标准、检定规程及本公司上级部门有关要求。
2.引用标准GB/T 17215-20021级和2级静止式交流有功电能表GB/T 17442-19981级和2级直接接入静止式交流有功电度表验收检验DL/T 645-1997多功能电能表通信规约JJG 596-1999《电子式电能表》检定规程3、技术要求3.1技术参数3.1.1电压Un:220V;工作电压X围:0.7Un~1.2Un,误差应符合等级要求,1.9Un时的条件下4小时电表不损坏。
3.1.2 基本电流为Ib:直接接入式:5(30)A、10(40)A、20(80)A。
在6Ib条件下连续运行,误差在等级X围内,仪表各部件不损坏。
3.1.3 参比频率:50Hz,频率X围:50Hz±5%3.1.4 准确度等级:2.0级(按50%误差限验收)。
3.1.5工作温度X围:-20℃~+55℃;工作极限温度X围:-25℃~+70℃;贮存与运输温度X围:-25℃~+70℃。
3.1.6功耗电压线路功耗:≤1W,5VA;电流线路功耗:≤1VA(Ib)。
3.2外观要求3.2.1铭牌应符合GB/T17215—2002中4.2.13.1规定,标志清晰,带有条形码的位置。
铭牌上应有计量器具生产许可证和制造标准的标识,铭牌材料宜采用铝板。
3.2.2表壳应有密封、防尘、防潮、防水性能,并具有一定的强度,由能抗变形、抗腐蚀、抗老化的阻燃材料制成,采用延伸型或嵌入型底座,采用II类防护绝缘包封。
3.2.3端钮盒内所有桩头一体化,螺钉采用“-”、“+”通用。
辅助接线端应处于低电侧。
电压、电流的接线柱在受到接线压力(60牛)时,辅助端的接线柱在受到接线压力(10牛)时接线柱不上缩。
DISCOVER A NEW EXPANSE OF COLOURL9H SERIES TRICHROMA LASER TVInspired by a world of incredible colour at a scale that makes everything feel brand new. The 3000 Lumen ultra-short throw projection TV features the TriChroma laser engine to reach 107% of the BT.2020 Colour space. L9H series is upgraded to support Dolby Vision, bringing incredibly vivid details to life. Premium features like Dolby Atmos® High-Speed HDMI, Filmmaker Mode, and a perfectly paired screen make the L9H the ultimate home entertainment upgrade.The triple colour laser light source generates three original colours (Red, Blue and Green) directly without a spinning colour wheel. Wider Colour Gamut and continuous improvements in the Trichroma laser engine and screen create images what the human eyes truly see in nature.107%BT.2020 Colour space1.07 BillionColoursMeet the L9H TriChroma Laser TVPremium X-FUSION™ Laser Light Source• Red + Green + Blue Lasers • 107% BT.2020 colour space • 3000 Lumens Brightness• 4K Resolution, 60Hz Refresh • Dolby Vision• 40W Dolby Atmos ® Sound• High-Speed HDMI with eARC • WiSA Ready • Google TVGet that childlike grin of excitement as you re-experience allyour favorites at a scale that makes everything feel brandnew. The massive 120-inch Laser TV display uses Ambient LightRejection technology to produce an incredibly bright picturethat’s enjoyable in any viewing environment, light or dark. This isyour true living room projection TV to take sports, streaming, andgaming to the next level.3000 Lumens. That’s right, the L9H shines with brilliance so allyour 4K HDR content hits those shimmering highlights, vibrantColours, and voluminous blacks that make everything pop off thescreen. And with incredible brightness uniformity across the entireprojection — no vignetting, no falloff — L9H is a shining wall of light.With built-in 40W Dolby Atmos sound, L9H is a big sound-stageupgrade over those tinny speakers in your last TV. Get clearspeech, thrilling highs, and booming lows without having toinvest in any extra gear. For the true audiophiles, high-speedHDMI with eARC allows for pass-thru of high-bitrate audio toyour surround sound system.120″Laser TV Display3000Lumens40WStereo Sound80%Ambient Light Rejection2,000,000:1Dynamic ContrastUltra High Speed HDMIwith eARCBig Screen ExperienceA Bright Picture in Any Room Powerful Sound2xUltra High Speed HDMI (1 with eARC)1xHDMI 2.01x 2xUSB1xDigital Audio Out 1xPortsAll product, product specifications, and data are subject to change without notice to improve reliability, function, design or otherwise. ©2023 Hisense Canada, All rights reservedHisense Canada Co., LtdUnit 1, 2550 Meadowvale Blvd, Mississauga, ON L5N 8C2 1-855-344-7367PhysicalConsole Dimensions 24” x 6.1” x 13.6” (W x H x D)Console Weight 24.7 lbsScreen Dimensions 104.6” x 60.4” x 1.4” (W x H x D)Screen Weight34.2 lbsOver box/Package Dimensions 69.1” x 25.7” x 20.1” (W x H x D)Over box/Package Weight110.3 lbsPictureProjection Size 120”Brightness3000 Lumens Resolution / Refresh Rate 4K@60Hz Colour Space 107% BT.2020Contrast Ratio 2,000,000:1 (dynamic)HDRHDR10, HLG, Dolby VisionLight Source Red + Green + Blue Trichroma Laser Laser Life 25,000+ Hours Throw Ratio 0.25:1Chipset0.47” DMDAudioAudio Output Power 40W (Stereo)Surround SoundDolby Atmos, Dolby DigitalSmart FeaturesSmart TV Platform Google TV (Google Certified)App StoreGoogle Play StoreStreaming Services Netflix, Disney+, HBO NOW, Hulu, Prime Video, SHOWTIME, Pandora, Sling TV, YouTube Voice Assistant Google AssistantWorks With Hey Google, Amazon Alexa Screen MirroringChromecast, AirPlay 2ConnectivityWi-Fi 802.11a/b/g/n/ac (Dual-Band, Wifi 6e)Bluetooth Yes Wired EthernetYesPowerPower Consumption 320W Standby Consumption <0.5W Power SupplyAC 120V, 60HzPortsHDMI2x HDMI 2.1 (ALLM), 1x HDMI 2.0HDMI ARC, CEC 1x (eARC on HDMI port 2)USB 1x USB 3.0, 1x USB 2.0RF Antenna 1x Ethernet (LAN)1x Digital Audio Output 1x Optical Analog Audio Output1xOther FeaturesNoise Reduction Yes Parental Controls Yes Closed Caption Yes Sleep Timer Yes Eye Safety Yes MEMCYes Remote FinderYesAccessoriesRemoteYes, voice remote with backlight Quick Start Guide / Manual QSG in box, Manual online Power Cable Yes Cleaning KitYesScreenScreen Size 120” diagonalTypeLenticular ALR (Ambient Light Rejecting)Resolution4K Ambient light obscuring ratio 85%Gain0.6Viewing Angle 150°Frame Fix (assembly required)Install Manual Yes Mounting Brackets Yes GlovesYesWarranty 2 Years limited warranty UPC/EANUPC: 888143014661EAN: 6942147488973Setup DiagramsH14 5/8”L111 7/8”W>90”H1>68”H261”Recommended TV stand height: <20”TECHNICAL SPECIFICATIONSLaser Model | 120L9H-DLT100C CONSOLETV STANDTV STANDH1(Minimum wall height)(mounting bracket holes)。
G-series Lua API 参考文档V8.50预览与概述手册目录手册目录 (2)内容预览 (3)参考文档 (4)标准 Lua 5.1 库 (40)附录A (41)G-series Lua API 参考手册是一套使用 Lua 脚本编程语言描述并且为 G-series 系列游戏键盘提供高级脚本功能。
该文档是在假设您已初步掌握 Lua 脚本编程语言使用方法的前提下进行的。
如果您想了解更多信息请访问 。
每项 G 系列的配置文件都有一个默认的 Lua 脚本与其绑定,并且您可以根据您的喜好随意编辑或者自定义。
该脚本借助并使用一个名为OnEvent 的事件句柄。
用户可以通过检查此句柄中触发的各种事件以执行用户所希望的动作。
方法列表OnEvent (5)GetMKeyState (7)SetMKeyState (8)Sleep (9)OutputLogMessage (10)GetRunningTime (11)GetDate (12)ClearLog (13)PressKey (14)ReleaseKey (15)PressAndReleaseKey (16)IsModifierPressed (17)PressMouseButton (18)ReleaseMouseButton (19)PressAndReleaseMouseButton (20)IsMouseButtonPressed (21)MoveMouseTo (22)MoveMouseWheel (23)MoveMouseRelative (24)MoveMouseToVirtual (25)GetMousePosition (26)OutputLCDMessage (27)ClearLCD (28)PlayMacro (29)AbortMacro (30)IsKeyLockOn (31)SetBacklightColor (32)OutputDebugMessage (33)SetMouseDPITable (34)SetMouseDPITableIndex (35)EnablePrimaryMouseButtonEvents (36)G13 Programming (37)OnEventOnEvent() 方法为脚本提供了一系列事件句柄以方便用户对触发的事件进行操作,您可以使用以下代码实现此方法。
DTS188型H1(H3)三相电子式电能表技术说明书V1.0编制:_____审核:_____批准:_____宁波三星科技有限公司2007年月日目录一产品简介二基本功能三显示及操作说明四安全防护和密码管理五外形说明及安装附录 A 默认初始参数表附录 B 主要元器件清单附录 C 功能附录表一、产品简介1、概述DTS188/DSS188型三相电子式电能表(以下简称“仪表”)由三相电子式电能表计量单元和数据处理单元组成,LCD显示,有红外和RS485通信功能。
该表具有测量精度高、稳定性好、过载能力强、可靠性高等显著优点。
是为电力部门提供的先进、可靠的计量工具。
该表型根据国家标准GB/T 17215-2002《1级和2级静止式交流有功电能表》设计,技术要求符合《浙江省三相液晶显示(非分时)电能表技术条件》;通讯规约符合DL/T 645-1997《多功能表通讯规约》、《浙江省单、三相电能表通信规约(V1.0)》及符合省局《RS-485红外通讯口的安全技术条件》要求。
2、规格型号注:可根据用户需要制造特殊规格的表。
3、主要电气性能指标3.1 基本误差:见表1表1 基本误差3.2 起动电流:有功≤0.005I b(2级)有功≤0.003I b(2级,经互感器接入)有功≤0.004I b(2级)有功≤0.002I b(1级,经互感器接入)。
3.3潜动:电压线路加115%Un,当电流回路无电流时,电能表输出的脉冲不多于1个。
3.4 电气参数3.5 时钟参数3.6 气候条件3.7 外型尺寸:249 mm×170 mm×75mm。
3.8 安全性能:交流绝缘电压试验:试验电压4kV,符合GB/T17215-2002要求。
冲击电压试验:冲击电压6 kV,通过EMC试验合格,符合GB/T 17215-2002要求。
1.9U n时的条件下4h电表不损坏。
4、产品原理框图说明如下:⑴、电量计量:单片机H8/38124采集电能计量芯片,计算出相应的功率,同时根据电表的脉冲常数,转换成电能的脉冲输出;再根据采集到的脉冲数,每计到0.01kWh电量的脉冲数,电量加0.01kWh.⑵、电源:电源采用变压器供电方式。
电容品牌大全主板厂商惯用电容品牌富士康Rubycon Sanyo华擎KZG KZE升技Rubycon技嘉Rubycon KZG OST磐正Sanyo OST GSC微星KZG OST华硕Nichicon KZG硕泰克Sanyo Sacon捷波GSC七彩虹Taicon按照Intel主板技术白皮书的介绍,主板CPU插槽附近的滤波电容单个容量最低要求为1000μF。
大部分主板上常见电容的容量为2200μF,好的主板采用3500μF甚至更高容量的电容。
而在Intel的原装主板上,一般单个电容容量都在3300μF以上,这就是Intel主板极其稳定的重要原因之一。
可见“电容决定主板质量”这话一点不假。
下面是主要电容品牌的体系图,都是从电容厂商的网站上DOWN下来的,买电容的时候可以参考一下:目前只找到SANYO、nichicon的体系图,其它厂商只提供PDF文档,有兴趣的可以去看:SANYO: www.secc.co.jp/english/index.htmlnichicon: chemicon: rubycon: teapo: OST: 总结一下,比较适合主板使用的电容必须是\"Low Impedance & ESR\"、\"Very Low Impedance & ESR\"、\"Ultra Low Impedance & ESR\" 且温度为105C的,Ultra的最好SANYO:MV-WG、MV-WX、MV-SWG、MB-UWG、MB-EXR等系列nichicon: H开头的系列,P开头的系列chemicon: KZE、KZG系列rubycon: YXF、YXG、ZL、ZLH、MBZ、MCZ系列teapo: SC、SM、SZ系列OST: RLP、RLZ系列系列太多了,列举不完。
在可选的系列中,再根据PDF的资料,选择寿命比较长的就好了,一般PC只需在2000小时以上就差不多了,5000小时以上的一是难买,二是贵。
Red Gi ant 红巨星系列插件(包括T rapco de系列,Magic Bulle t系列等)注册码Tra pcode系列插件 32位/64位 AE7.0 c s3 cs4 cs5已测试注册成功Trap code3dstr oke v2.6.08504-8786-2500-7973-4045Tr apcod e Ech o Spa ce v1.1.01806-9363-1382-3577-4294Trap codeform v1.1.08963-8983-1000-6183-3028T rapco de ho rizon v1.1.08407-9462-1000-8739-6032Trapc ode L ux v1.1.09351-9185-1115-4017-51058813-9153-1391-3563-82938901-9141-1535-5036-4525Trap codeparti cular v2.1.08476 8810 2111 5743 91408291 8812 2111 9741 5955T rapco de sh ine v1.6.0 (注意:先装演示版再在A ECS5中注册)9018-8522-1500-3971-1535Trapc ode S oundkeysv1.2.01406-9268-1890-7019-3890Tr apcod e Sta rglow v1.6.09122-8674-1500-6827-0151=========================================RedGiant Magi c Bul let 系列插件 32bi t 64b it 注册码Ma gic B ullet Mojov1.1/v1.28450 1630 1000 4153 01398357-1608-1000-4258-9046Magic Bull et Lo oks v1.4.1/v1.4.38335 2967 1000 7517 96809443-2929-1498-8572-38362226-2975-1561-7392-91258946-2914-1090-9732-7381Magic Bull et Co loris ta Ⅱ调色师1759 1720 1942 8719 98951520-1761-1281-4491-29951623-1704-1894-4141-77111669-1738-1942-6448-38052077-1774-1115-5675-2386M agicBulle t Ins tantH D v1.1/v1.2 视频转换8680-8082-1000-3600-21378374-8050-1000-7790-58318651-8096-1000-8165-81088341-8036-1000-6446-8798======================================== RedGiant红巨星其它插件 32b it 64bit 注册码R ed Gi ant K ey Co rrect v1.1/v1.2抠像校正8884 7072 1000 6861 92218831-7014-1000-7750-71688524-7000-1000-5504-98618932-7028-1000-7300-8269Red G iantKnoll Ligh t Fac tory2.7 灯光工厂9248-3000-2939-3565-1044Red Gian t Pla neSpa ce v1.4 3D助手2272-5113-1995-5538-18691785-5150-1912-5669-62991555-5174-1711-8253-68681728-5161-1306-8162-8636R ed Gi ant I mageLoung e v1.4 烟雾水火9601-7161-1124-7096-5124Red Gian t Psu namiv1.3.1/v1.4 天空海洋8918-5306-1340-8021-98918755-5319-1340-9790-27288695-5367-1340-8236-86688539-5315-1340-9036-6512R ed Gi ant W arp v1.1 三维变形反射投影2264-0345-1839-7036-81291530-0325-1394-5601-09502072-0325-1600-5426-96981732-0305-1823-5049-0581RedGiant Text Anar chy v2.4 极品文字特效8418-5446-2340-7452-29038630-5454-2340-8133-7115Re d Gia nt Ho lomat rix v1.2 屏幕视觉效果1772-6157-1942-4827-54361841-6194-1153-6422-8716R ed Gi ant P rimat te Ke yer v4.1 极品抠像8528-4002-4000-9517-05058783-4068-4000-6068-87609150-4038-4000-9260-21278704-4062-4000-7287-2681Re d Gia nt To onItv2.1卡通、水墨、油画2224-5529-2995-5538-18691737-5574-2912-5669-62991456-5541-2035-8665-5141R ed Gi ant C ompos ite W izard 1.4.5 1558-7224-1340-6093-82528338-7202-1384-7715-20839116-7278-1571-5973-40481377-7270-1729-7167-94601695-7274-1450-8641-5499******************************************************* 附:旧版 Red Gian t红巨星系列插件注册码R ed Gi ant C ompos ite W izard v1.4seri al: 1558-7224-1340-6093-8252After Effe cts 6.0-8.0 Sup portRed Gian t Ima ge Lo ungev1.4seria l: 9601-7161-1124-7096-5124A fterEffec ts 6.0-8.0 Supp ort RedGiant Knol l Lig ht Fa ctory Prov2.5.2ser ial:9248-3000-2939-3565-10448405-3024-2555-8703-3817Af ter E ffect s, Pr emier e Pro,Fina l Cut Pro,Motio n, Av idRed.G iant.Prima tte.K eyer.Pro.v4.0.f or.AE.and.AVXs erial: 9473-4044-4228-6877-3228Af ter E ffect s, Av id,Fi nal C ut Pr o, an d Mot ionRed G iantRadiu m Glo w v1.0ser ial:1374-0436-1563-3568-3475FCP6.x下使用有退出状况,建议使用前保存FC P工程文件。
Ruby语言介绍
目录
Ruby语言介绍 (1)
目录 (1)
1.基本的ruby语法 (2)
1.1变量、常量和类型 (2)
1.2注释 (2)
1.3循环和分支 (2)
1.4正则表达式 (4)
2,常用函数 (7)
2.1Numeric 类 (7)
2.2Float 类 (7)
2.3String 类 (7)
2.4Array 类 (9)
2.5Hash 类 (11)
1.基本的ruby语法
1.1变量、常量和类型
1)定义变量
2)变量内插
在双引号内使用“#(变虽名}"内插变最
1.2注释
1)单行注释:以#开头,如:#注释内容
2)多行注释:在=begin和=end之间定义,如: =begin
注释内容
=end
13循环和分支
1.3.4 类
135模块
方法说明
include,watir' 将模块的功能添加到当前空间中,不加载己加载的文件
1.3.6 case 语句case x
1.4正则表达式
1.4.1普通字符
普通字符由字母、数字、下划线和特殊符号组成。
4/13
如:
表达式/b/在匹配字符“abed”时,匹配的内容是“b” 表达式/b_/在匹配字符"ab_cd”时,匹
配的内容是“b_”
1.4.2转义符
1.4.3匹配多种字符
1.4.4修饰匹配次数的方法
1-4.5匹配模式
2.常用函数2.1 Numeric 类
2. 4 Array 类
2.5 Hash 类。
红宝石电容英文名称:Rubycon生产公司:日本RUBYCON CORPORATION。
其是具有四十多年专业生产电解电容的专业公司,生产管理严格,产品质量稳定。
用于生产电解电容的主要原材料铝箔和电解液均为RUBYCON CORPORATION自己研制、生产,有效地保证了产品质量。
RUBYCON作为电解电容国际著名品牌,已在国内的节能灯、镇流器、电表、开关电源等领域产品上广泛使用。
主要电容系列产品:通讯专用系列:贴片电解SEV-40~85℃、SGV(2000小时)-40~105℃电表专用系列:YXA、YXF(高频长寿命)-55~105℃节能灯专用系列:YXA、BXA(1000~12000小时)、BXC(5000~1000小时)、CFX(5000小时)-55!105℃开关电源专用系列:YXA、YXF、YXG、MXR、MXG、MXC、AXW、WXA、ZL、ZLH-40~85℃标准系列:YK、USR -40~85℃在个人电脑和音响领域,使用红宝石电容的产品被广泛的认为是优质的。
★REV系列(85度贴片标准品)、RKV系列(105度贴片高温品):用于数码相机、IC卡电话机、随身听、电话计费器、电脑及DVD板卡、汽车电器等高可靠性的袖珍电子产品中.★ZAV系列(105度贴片超低阻抗品):在电性能上与钽电解电容相当,是贴片钽电解电容的替代品,而成本上远低于钽电解电容.★YK系列(85度普通标准品)、YXA系列(105度高温标准品):应用于家用电器、电脑板卡、仪表、消费类电子产品等,在可靠性高的场合,宜采用YXA系列.★PK系列(85度超小型品)、PX系列(105度超小型品):电性能上分别与YK系列、YXA系列相对应,而体积上比前者缩小了近百分之四十,特别适合用于小体积的电子产品中.★YXF系列(105度长寿命、低阻抗品)YXG系列(105度长寿命、高纹波品):用于开关电源作为输出滤波用,在滤波要求高的场合建议采用YXG系列,长寿命数字电能表建议采用YXF系列.★ZA系列(105度超低阻抗品)、ZL系列(105度高纹波低阻抗品):是YXF、YXG的改进型,在耐纹波的能力上有很大提高,特别适用于军事、航天、航空等领域的开关电源中.★MS5系列(85度普通品)、MH5(105高温品)用于微型电子产品.★BXA系列(105度8000-10000小时长寿命品)、CFX系列(105度5000小时长寿命品):节能灯及电子镇流器专用品.★USR系列(85度针脚基板自立型)、MXR系列(105度过针脚基板自立型):彩电、显示器、空调等电子产品中工频电源部分滤波用.★LSQ系列(85度针脚螺丝锁大电解)、LSW系列(105度螺丝锁大电解):用于大功率工频电源设备中,如:工业变频器、大型不间断电源等等.WXA、AXW系列(105度小型品):小型电源专用品.★WXF系列(105度15MM高基板自立型):PDP、LCD等扁平显示器专用品.1.http://www.rubycon.co.jp/cn/官方网站2./product.asp?BigClassName=红宝石电容红宝石规格书下载。
常用电解电容型号电解电容是一种常见的电子元器件,广泛应用于各种电子设备中。
它具有容量大、体积小、价格低廉等特点,因此在电子领域得到了广泛的应用。
下面将介绍一些常用的电解电容型号及其特点。
1. 电解电容型号:ELNA RE3系列特点:ELNA RE3系列电解电容具有高温工作能力,适用于高温环境下的电子设备。
该系列电容器采用铝质电解质,容量范围从1μF到470μF不等,电压范围从6.3V到100V。
此外,RE3系列电容器具有低阻抗、低ESR和长寿命等特点。
2. 电解电容型号:Nichicon HE系列特点:Nichicon HE系列电解电容适用于高温高湿度环境下的电子设备。
该系列电容器采用高温高湿度耐受性电解质,容量范围从1μF到470μF不等,电压范围从6.3V到100V。
HE系列电容器具有低频特性好、低ESR和长寿命等特点。
3. 电解电容型号:Panasonic FC系列特点:Panasonic FC系列电解电容器是一种高性能电容器,适用于各种电子设备。
该系列电容器采用高品质的电解质和极片材料,容量范围从1μF到1000μF不等,电压范围从6.3V到100V。
FC 系列电容器具有低ESR、低泄漏电流和长寿命等特点。
4. 电解电容型号:Rubycon YXF系列特点:Rubycon YXF系列电解电容是一种高质量的电容器,适用于各种电子设备。
该系列电容器采用高品质的电解质和极片材料,容量范围从1μF到1000μF不等,电压范围从6.3V到100V。
YXF 系列电容器具有低ESR、低泄漏电流和长寿命等特点。
5. 电解电容型号:Nippon Chemi-Con KZE系列特点:Nippon Chemi-Con KZE系列电解电容是一种高性能电容器,适用于各种电子设备。
该系列电容器采用高品质的电解质和极片材料,容量范围从1μF到1000μF不等,电压范围从6.3V到100V。
KZE系列电容器具有低ESR、低泄漏电流和长寿命等特点。
查询PSP100B-5.0R(中文)供应商捷多邦,专业PCB打样工厂,24小时加急出货PSP100B 宽输入电压范围DC-DC电源数据手册VER 1.0 2002年6月PSP100B宽输入电压范围DC-DC电源数据手册武汉力源电子股份有限公司二零零二年六月PSP100B宽输入电压范围DC-DC电源数据手册VER 1.0 2002年6月PSP100B宽输入电压范围降压型DC-DC电源数据手册一、功能说明PSP100B系列宽输入电压降压型DC-DC变换模块是一种工作频率为150KHz的开关型电源。
它可将4.5V∽40V的直流输入电压变换为3.3V、5V、12V等,输出电流高达1A,5V输出产品的典型变换效率为80%。
由于设计工作频率高达150KHz,因而整个电源的体积较小最大输出功率可达12W。
工作温度范围为-20℃∽85℃。
PSP100B具有体积小、重量轻、使用方便和性价比高的特点。
因此,是各种小型电子设备、车载设备,特别是需用电池作为后备电源供电的电子产品的理想选择。
二、引脚及外形尺寸1、引脚说明PSP100B元器件排列如图1所示,各引脚的功能见表1。
EN Vin GND Vout图1 PSP100B元器件排列图表1 PSP100B引脚功能符号功能说明EN 使能控制端,接低电平电源正常工作,接高电平电源进入备用状态GND 输出电压地,这些引脚同输入电压地脚内部相连V out输出电压引脚Vin 输入电压引脚2、外型尺寸PSP100B电源采用铝基板单面贴装工艺,具有良好的散热性能,引出线为单排直插式,既SIP结构。
PSP100B外型尺寸为35 x 30 x 12.5 mm3,图2是PSP100B的外形尺寸图。
引出脚与78xx系列线性稳压器兼容。
(4个单排针引脚的实际长度为5+0.5mm)PSP100B宽输入电压范围DC-DC电源数据手册VER 1.0 2002年6月图2a PSP100B电源模块的正视图图2b PSP100B电源模块的侧视图三、技术指标1、技术参数PSP100B主要技术参数见表2,其中典型值为设计参数,最大最小值是由出厂检测保证的参数。
Philips MonitorUSB-C monitor5000 series27" (68.6 cm)2560 x 1440 (QHD)27E1N5600AESimplify your connectionswith USB-CThis Philips USB-C multipurpose monitor offers clutter-free setup. With a single cable youcan view smooth high-resolution video, transfer data, and charge your laptop. A true hubfor mobile professionals.Single cable USB-C connection•Connect your notebook with one USB-C cableSuperb Picture Quality•IPS LED wide view technology for image and color accuracy•Crystalclear images with Quad HD 2560 x 1440 pixels•1ms (MPRT) fast response for crisp image and smooth gameplay•SmartImage game mode optimized for gamers•SmartContrast for rich black detailsFeatures designed for you•LowBlue mode and Flicker-free easy-on-the eyes viewing•Built-in stereo speakers for multimedia•Tilt, pivot and height-adjust for an ideal viewing position•EasyRead mode for a paper-like reading experience•Cable management reduces cable clutter for neat workspace•EasySelect menu toggle key for quick on-screen menu accessHighlightsUSB-C connectionThis Philips display features a USB type-C connector with power delivery. Withintelligent and flexible power management, you can power charge your compatible device directly. Its slim, reversible USB-C allows for easy, one-cable connection. You can watch high resolution video and transfer data at a super-speed, while powering up and re-charging your compatible device at the same time.1ms MPRT fast responseMPRT (motion picture response time) is more intuitive way to describe the response time, which directly refers the duration from seeing blurry noise to clean and crisp images. Philips gaming monitor with 1 ms MPRT effectively eliminates smearing and motion blur, delivers shaper and precise visuals to enhance gaming experience. Best choice for playing thrilling and twitch-sensitive games.Crystalclear imagesThese Philips screens deliver Crystalclear, Quad HD 2560x1440 or 2560x1080 pixel images. Utilizing high performance panels with high density pixel count, enabled by high bandwidth sources like USB-C, Displayport, HDMI, these new displays will make your images and graphics come alive. Whether you are demanding professional requiringextremely detailed information for CAD-CAM solutions, using 3D graphic applications or a financial wizard working on huge spreadsheets, Philips displays will give you Crystalclear images.SmartImage Game modeThe new Philips gaming display has quick access OSD fine tuned for gamers, offering you multiple options. "FPS" mode (First person shooting) improves dark themes in games, allowing you to see hidden objects in dark areas. "Racing" mode adapts display with fastest response time, high color, along with image adjustments. "RTS" mode (Real time strategy) has a special SmartFrame mode which enables highlighting of specific area and allows for size and image adjustments. Gamer 1 and Gamer 2 enable you to save personalcustomized settings based on different games, ensuring best performance.LowBlue Mode & Flicker-freeOur LowBlue Mode and Flicker-freetechnology have been developed to reduce eye strain and fatigue often caused by long hours in front of a monitor.Built-in stereo speakersA pair of high quality stereo speakers built into a display device. It can be visible front firing, or invisible down firing, top firing, rear firing, etc depending on model and design.Compact Ergo BaseThe Compact Ergo Base is a people-friendly Philips monitor base that tilts, pivot and height-adjusts so each user can position the monitorfor maximum viewing comfort and efficiency.Issue date 2023-09-13 Version: 3.1.112 NC: 8670 001 80248 EAN: 87 12581 78340 2© 2023 Koninklijke Philips N.V.All Rights reserved.Specifications are subject to change without notice. Trademarks are the property of Koninklijke Philips N.V. or their respective owners.SpecificationsPicture/Display•LCD panel type: IPS technology•Backlight type: W-LED system•Panel Size: 27 inch / 68.6 cm•Display Screen Coating: Anti-Glare, 3H, Haze 25%•Effective viewing area: 596.736 (H) x 335.664 (V)•Aspect ratio: 16:9•Maximum resolution: 2560 x 1440 @ 75 Hz*•Pixel Density: 108.79 PPI•Response time (typical): 4 ms (Gray to Gray)*•MPRT:1ms•Brightness: 300 cd/m²•Contrast ratio (typical): 1000:1•SmartContrast: Mega Infinity DCR•Pixel pitch: 0.2331 x 0.2331 mm•Viewing angle: 178º (H) / 178º (V), @ C/R > 10•Flicker-free•Picture enhancement: SmartImage game •Color gamut (typical): NTSC 102.2%*, sRGB 114.18%*•Display colors: 16.7 M•Scanning Frequency: 30 - 114 kHz (H) / 48 - 75 Hz (V)•LowBlue Mode•EasyRead•sRGB•Adaptive syncConnectivity•Signal Input: HDMI 1.4 x 1, DisplayPort 1.2 x 1, USB-C 3.2 Gen 1 x 1 (upstream, Power Delivery up to 65W)•HDCP: HDCP 1.4 (HDMI / DisplayPort / USB-C)•USB:: USB 3.2 x 4 (downstream with 1 fast charge B.C 1.2)•Sync Input: Separate Sync•Audio (In/Out): Audio outUSB•USB-C: Reversible plug connector•Super speed: Data and Video transfer•DP: Built-in Display Port Alt mode•Power delivery: USB PD version 3.0•USB-C max. power delivery: Up to 65W (5V/3A, 9V/3A, 10V/3A, 12V/3A, 15V/3A, 20V/3.25A) Convenience•Built-in Speakers: 3 W x 2•User convenience:Power On/Off, Menu, Volume, Input, SmartImage Game•OSD Languages: Brazil Portuguese, Czech, Dutch, English, Finnish, French, German, Greek, Hungarian, Italian, Japanese, Korean, Polish, Portuguese, Russian, Simplified Chinese, Spanish, Swedish, Traditional Chinese, Turkish, Ukrainian •Other convenience: Kensington lock, VESA mount(100x100mm)•Plug & Play Compatibility: DDC/CI, Mac OS X,sRGB, Windows 10 / 8.1 / 8 / 7Stand•Height adjustment: 130 mm•Pivot: -/+ 90 degree•Tilt: -5/20 degreePower•On mode: 29.1 W (typ.)•Standby mode: 0.5 W (typ.)•Off mode: 0.3 W (typ.)•Energy Label Class: F•Power LED indicator: Operation - White, Standbymode- White (blinking)•Power supply: Internal, 100-240VAC, 50-60HzDimensions•Product with stand(max height): 614 x 510 x212 mm•Product without stand (mm): 614 x 365 x 52 mm•Packaging in mm (WxHxD): 780 x 525 x 186 mmWeight•Product with stand (kg): 6.56 kg•Product without stand (kg): 4.70 kg•Product with packaging (kg): 10.64 kgOperating conditions•Temperature range (operation): 0 to 40 °C•Temperature range (storage): -20 to 60 °C•Relative humidity: 20%-80 %•Altitude: Operation: +12,000ft (3,658m), Non-operation: +40,000ft (12,192m)•MTBF: 50,000 hrs (excluding backlight) hour(s)Sustainability•Environmental and energy: RoHS•Recyclable packaging material: 100 %•Specific Substances: Mercury free, PVC / BFR freehousingCompliance and standards•Regulatory Approvals: CB, CE Mark, CU-EAC,EAEU RoHS, FCC Class B, ICES-003, TUV/ISO9241-307, TUV-BAUARTCabinet•Color: Black•Finish: TexturedWhat's in the box?•Monitor with stand•Cables: HDMI cable, USB-C to USB-C cable,Power cable•User Documentation*"IPS" word mark / trademark and related patents on technologiesbelong to their respective owners.*The maximum resolution works for either HDMI input or DP input.*For best output performance, please always ensure that your graphiccard is capable of achieving the maximum resolution and refresh rateof this Philips display.*Response time value equal to SmartResponse*MPRT is to adjust brightness for blur reduction, so cannot adjustbrightness while MPRT is turned on. To reduce motion blur, LEDbacklight will strobe synchronously with screen refresh, which maycause noticeable brightness change.*MPRT is gaming-optimized mode. Turn on MPRT may causenoticeable screen flickering. It is recommended to turn off when youare not using the gaming function.*NTSC Area based on CIE1976*sRGB Area based on CIE1931*For USB-C power and charging function, your Notebook/devicemust support USB-C standard Power Delivery specifications. Pleasecheck with your Notebook user manual or manufacturer for moredetails.*For Video transmission via USB-C, your Notebook/device mustsupport USB-C DP Alt mode*The monitor may look different from feature images.。
Product InsertCatchPoint ™ Cyclic-GMP Fluorescent Assay Kit Product # R8075 (Bulk Kit) Quantity: 960, 120 µL reactionsIntroductionAbout the cGMP AssayThe CatchPoint ™ cyclic-GMP Fluorescent Assay Kit measures levels of 3’, 5’-cyclic guanosine monophosphate (cGMP) or guanylate cyclase activity via a competitiveimmunoassay for cGMP. The assay requires only a single washing step, and readings can be taken in as little as 10 minutes or as long as 24 hours following substrate addition, since no termination step is needed. The cGMP Fluorescent Assay Kit is suitable for use in cell-based assays.Principle of the AssayThe cGMP in the sample or standard competes with horseradish peroxidase (HRP)-labeled cGMP conjugate for binding sites on the anti-cGMP antibodies (Fig. 1). In theabsence of cGMP, most of the HRP-cGMP conjugate is bound to the antibody. Increasing concentrations of cGMP competitively decrease the amount of bound conjugate, thus decreasing measured HRP activity.Figure 1. Principle of the CatchPoint cGMP assay systemApplicationsThe kit is designed for use in applications where cGMP is generated in biochemical assaysystems, such as assays of guanylate cyclase activity. In addition, this kit can be used forcell-based assays to measure intracellular cGMP levels following stimulation with peptidehormones or stimulants such as nitric oxide (NO), sodium nitrate, and nitroprusside tosoluble or particulate guanylate cyclases.Materials and EquipmentKit Components The following table lists the kit components.Table 1: The CatchPoint cGMP Fluorescent Assay Kit (P/N R8075) contentsReagent Quantity Description96-well black plates (clear bottom) 10 plates 96-well plate coated with Goat anti-Rabbit IgG. Plates are pre-blocked andready to use.cGMP Assay Buffer 950 mLRabbit anti-cGMP Antibody 2 vials Antibody, lyophilizedcGMPCalibrator2 vials30 µM cGMP Calibrator, lyophilized.On reconstitution with 5 mL assaybuffer each vial contains 30,000 pmolcGMP / mL.HRP-cGMPConjugate2 vials HRP-cGMP Conjugate, lyophilized10X Wash Concentrate 950 mLCell Lysis Buffer 450 mLStoplight Red Substrate 2 x 900 µL 100X Substrate in DMSOSubstrate Buffer 950 mLWhen the working concentrations of the above reagents are used as suggested, each kitprovides sufficient reagents for ten96-well microplates with 120 µL total assay volume.Storage and Handling All kit components are to be stored at 4°C.IMPORTANT: Allow all the reagents to warm to room temperature prior to use.Note: Small volumes of product will occasionally collect in the cap of the product vial during shipment. Gently tap the vial on a hard surface or briefly centrifuge the vial to collect any liquid trapped in the vial cap.When stored properly, the kit components are stable for six months from the date of receipt.The reconstituted working solutions of Rabbit anti-cGMP, cGMP Calibrator, and HRP-cGMP Conjugate are stable for 3 weeks at 4°C. To insure optimal performance of the reagents do not store below 4°C once reconstituted.If the entire plate will not be used, protect any unused wells with a plate sealer and store at 4°C in the original foil wrapper protected from light.IMPORTANT: The reconstituted substrate solution is sensitive to light. To insure optimal performance we recommend preparing a fresh stock solution (keep protected from light) and adding to the assay plate within 60 minutes. Following substrate addition readings can be taken at 10 minutes or as long as 24 hours.Materials Required but not Provided The following tables list the materials required but not supplied.Table 2: Reagents and suppliesReagent Item Source3% wt / vol hydrogen peroxide (H2O2) Major laboratory suppliers (MLS) 1.5 mL polypropylene cappedcentrifuge tubesMLS10 mL capped centrifuge tubes MLSPipettors – adjustable volume MLSMulti-channeltroughs MLSTable 3: Compatible Instruments available from Molecular Devices (MDC) Equipment Item SourceAnalyst SystemOne of the following:- Analyst GT- Analyst HT Gemini XPSGemini EMSpectraMax® M2 SpectraMax® M5 FlexStationEmbla 96/384 plate washer AquaMax DW4 with 96-well MDC P/N 0200-6003 or 0200-6004MDC P/N 0200-6043 or 0200-6044MDC P/N Gemini XPS SpectrophotometerMDC P/N Gemini EM SpectrofluorometerMDC P/N M2MDC P/N M5MDC P/N FlexStationII Scanning Fluorometer MDC P/N 0200-3948MDC P/N AquaMax DW4 Liquid Handling SystemCatchPoint cGMP Fluorescent Assay Kit Experimental ProtocolPreparing the Reagents The following protocol provides sufficient reagents to run five 96-well plates, each with 16 calibrators, 2 controls, and up to 78 samples.Table 4: Preparation of reagentsStep Action1 Reconstitute one vial of 30 µM cGMP Calibrator:Add 5 mL of cGMP Assay Buffer to the vial. Mix wellto ensure dissolution of all contents. Store on ice or at 4°C.2 Reconstitute one vial of HRP-cGMP Conjugate:Add 10 mL of cGMP Assay Buffer to one of the vials for a 10X stock.Mix well to ensure dissolution of all contents. Transfer the entirecontents of the vial to a 100 mL graduated cylinder by repeatedwashings with assay buffer. Adjust the final volume to 100 mL withassay buffer and mix thoroughly. The diluted HRP-cGMP conjugate isready for use in the assay. Store on ice or at 4°C.3 Reconstitute one vial of Rabbit anti-cGMP Antibody:Add 10 mL of cGMP Assay Buffer to one of the vials for a10X stock.Mix well to ensure dissolution of all contents. Transfer the entirecontents of the vial to a 100 mL graduated cylinder by repeatedwashings with assay buffer. Adjust the final volume to 100 mL withassay buffer and mix thoroughly. The diluted Rabbit anti-cGMPconjugate is ready for use in the assay. Store on ice or at 4°C.4 Dilute 10X Wash Concentrate 10-fold in deionizedwater, i.e. for one assay plate dilute 500 mL of 10X Wash Concentratein 4500 mL of deionized water.*5 Prepare calibrators: The 30 µM cGMP calibrator is diluted in cGMPAssay Buffer to prepare stock calibrators of 10000, 100, 33, 11, 3.7,1.2, and 0.4 nM cGMP. These give 3300, 33, 11, 3.7, 1.2, 0.41, and0.14 nM (400, 4.0, 1.3, 0.44, 0.15, 0.049, and 0.016 pmol) finalconcentrations in the assay.1. Add 500 µL of the 30 µM cGMP calibrator to 1000 µL of cGMPAssay Buffer. This is the 10,000 nM stock calibrator.2. Add 20 µL of the 10,000 nM stock calibrator to 1980 µL of cGMPAssay Buffer. This is the 100 nM stock calibrator.3. In cGMP Assay Buffer, serially dilute the 100 nM stock calibrator ina 3-fold fashion five times to produce the 33, 11, 3.7, 1.2, and 0.4nM cGMP stock calibrators. 40 µL of each calibrator are requiredper replicate. Prepare 1000 µL of each (ample for 10 replicates).As a 0 nM (zero dose) calibrator, use the cGMP Assay Buffer itself. * On dilution of the 10X Wash Buffer concentrate, the reagent contains 0.02 M Tris, 150 mM NaCl, 0.05% Tween 20, and 0.05% Proclin 200 (pH 7.4).Preparing the Plate For ease of analysis and calculation, the assay plate contains the calibrators, controls,and samples as outlined below. Use the suggested template (Table 5) and run thesamples in duplicate.Note: The order of addition of each component to the wells and the incubation time are important. Mix samples and all reagents gently but thoroughly before use.All assay wells will be prepared by dispensing the assay components in the following order:1) Calibrator, control, or sample2) Rabbit anti-cGMP Antibody3)HRP-cGMPConjugateTable 5: To prepare the assay plate:Step ActionDesigning your assay plate (plate map):We suggest preparing the plate according to the template below. If youare using SoftMax® Pro to analyze your results, you may set up atemplate before or after reading the plate. If you set up the plate before,you can print out a template to help in preparing your plate.ColumnRow 1 2 3A 3300 nM 3300 nM Control 1(400 pmol) (400 pmol)B 33 nM 33 nM Control 2(4 pmol) (4 pmol)C 11 nM 11 nM(1.3 pmol) (1.3 pmol)D 3.7 nM 3.7 nM(0.44 pmol) (0.44 pmol)E 1.2 nM 1.2 nM Column 3 (C –H) and(0.15 nM) (0.15 nM) all of columns 4 - 12:F 0.41 nM 0.41 nM Samples for analysis(0.049 pmol) (0.049 pmol)G 0.14 nM 0.14 nM(0.016 pmol) (0.016 pmol)H 0 nM 0 nM(buffer only) (buffer only)12 Add 40 µL of the appropriate concentration of Calibrator workingsolution (see step 5 from “Preparing the Reagents” on Table 4, page 4)to columns 1 and 2 (rows A – H). Column 3 (A & B) is designated herefor optional controls. For example, one may wish to use a zero dosecalibrator with no Rabbit anti-cGMP Antibody to evaluate background.Table 5: To prepare the assay plate:3 Place 40 µL of samples to be analyzed in appropriate wells [e.g.Column 3 (C – H), and columns 4 – 12]. See Appendix A for cellstimulation and lysis protocols.4 Add 40 µL of reconstituted Rabbit anti-cGMP Antibody to all wellsexcept any reserved for no antibody controls. If these are to beperformed, add 40 µL assay buffer in place of antibody.5 Place plate on shaker for 5 minutes or gently agitate by hand to ensuremixing.6 Add 40 µL reconstituted HRP-cGMP Conjugate to every well.7 Mix well and allow to incubate 2 hours at room temperature.8 Aspirate plate contents and wash 4 times with 300 µL wash bufferfor each wash.9 Prepare Stoplight Red Substrate: Dilute 600 µL of 100X stock StoplightRed Substrate into 60 mL of Substrate Buffer, then add 68 µL of 3%H202 (880 mM) to bring the final concentration to 0.0034% (1 mM) H202.Important: The reconstituted substrate solution is sensitive to light. Toinsure optimal performance we recommend preparing a fresh stocksolution and adding directly to assay plate within 60 min. At all timeskeep substrate protected from light.10 Add 100 µL Stoplight Red substrate to every well, minimizing the timebetween starting and finishing. Cover the plate and leave at roomtemperature for at least 10 minutes, shielded from light.11 Read the fluorescence intensity of the plate on an appropriateinstrument (see below for settings). Plates may generally be readanytime between 10 minutes and 24 hours. For optimal performanceyou may want to briefly mix the plate prior to reading.Reading the Plate in Analyst HTS Assay Detection SystemAnalyst ParameterSettingsThe following instrument settings are recommended when using 96-well plates with a100 µL volume in Analyst HTS systems:Table 6: Fluorescence intensity settings for Analyst HT or GT Detection System:Parameter SettingMode Fluorescence IntensityExcitation filters 530-25 nmEmission filters 590-20 nmDichroic mirror 50 / 50 beamsplittermmZ-height 3Attenuator MediumIntegration time 50,000 µsecLamp ContinuousReadings per well OnePMT setup Smart Read+ (sensitivity = 2)Units Counts / secReading the Plate in the Gemini XS, Gemini XPS, Gemini EM, SpectraMax® M2 andSpectraMax® M5 Detection SystemThe following instrument settings are recommended when using 96-well plates with aGemini XS system:Table 7: Fluorescence intensity settings for GEMINI XS, Gemini XPS, Gemini EM,SpectraMax® M2 and SpectraMax® M5 Detection SystemParameter SettingRead Mode EndpointRead Type Fluorescence (RFUs)Excitation 530 nmEmission 590 nmCutoff 570 nmSensitivity 6PMT AutoAutoCalibrate OnData Analysis Analyzing the Calibration DataThe average intensity values (y-axis) can be plotted against calibrator concentration (x-axis)to create a calibration or standard curve. Unknown samples can be interpolated using thiscurve to calculate cGMP doses in the samples. We recommend using SoftMax Pro softwarefor accurate curve fitting and interpolation of data from MDC instruments. Figure 2 shows aSoftMax Pro template and calibration curve for the cGMP assay when read on the Analyst™.Figure 2. Calibration curve for the CatchPoint cyclic GMP Fluorescent Assay Kit, read on the Analyst system. Data was taken 60 minutes after addition of Stoplight Red substrate. Fluorescence intensity settings for Analyst HTS Detection System were as stated in Table 6. The EC50 value of the calibration curve, calculated as coefficient C using SoftMax Pro, was 2.2 nM (264 fmol).ADDITIONAL INFORMATIONThe following typical information is based on data from an Analyst HT. Absolute readings may differ on other instruments, but relative values should be comparable.Sensitivity (Typical values)Minimum Detectable Concentration = 0.2 nM (24 fmol / well)Maximum Measurable Concentration = 120 nM (14 pmol / well)EC50 = 2.5 + 0.6 nM (300 fmol)Intra-assay precisionIntra-assay precision was calculated from measurements of 0 and 1.2 nM calibrator. The resultsfor a single assay are shown below:Signal intensity / background (for 0 nM calibrator) §Signal intensity Avg / background = 290 Standard deviation (SD) = 12%CV = 4n = 23 %B/B o* precision for 1.2 nM calibrator %B/B o (mean) = 59Standard deviation (SD) = 4%CV = 7n = 23§ Background taken as the signal intensity for wells in which no cGMP-HRP or Rabbit anti-cGMPAntibody are added* %B/B o = 100 X (I 1.2 nM – I ∞) / (I o – I ∞)whereI1.2 nM= Signal intensity for 1.2 nM calibratorIo= Signal intensity for 0 nM calibratorI∞= Signal intensity for3333 nM calibratorInter-assay precision (three assays performed on separate days)Inter-assay precision was calculated from measurements of %B/B o for 1.2 nM calibrator in 3 successive assays. The results are shown below:Measurement Data points %B/B0SD %CVA n = 2 58 5 8B n = 2 61 4 6C n = 23 59 4 7Precision correlation22 replicates of each of the standards were prepared and % coefficient of variation was determined for each concentration using SoftMax Pro.Sample ConcentrationMean ValueSD %CVcal 01 cal 02 cal 03 cal 04 cal 05 cal 06 cal 07 cal 08 3300 nM 33 nM 11 nM 3.7 nM 1.2 nM 0.41 nM 0.14 nM 0 nM 2084701994030487655910220036170454962204537524362726285929647826 135129 202898 447378 808640 982043 1233519 1049992 3.86.8 4.2 4.4 4.7 4.5 5.1 3.7Appendix A: Preparing cell lysates for the cGMP AssayThe protocol below illustrates an application of the CatchPoint cGMP Fluorescent Assay. The protocol has been developed for use with adherent cells stimulated with atrial natriuretic peptide (ANP). The procedure may be modified for various hormones and stimulators, such as acetylcholine, insulin, serotonin, and nitric oxide.For best results each cell line should be evaluated to determine the optimal concentration of cells per well, incubation times, and concentration of guanylate cyclase agonists or antagonists.Use this protocol as a guide to help optimize the assay.______________________________________________________________________________________ReagentsReagent Stock ConcentrationcGMP stimulator atrial natriuretic peptide, dissolved in phosphate buffered saline (PBS)300 µM Phosphodiesterase inhibitor, 3-isobutyl-1-methylxanthine (IBMX) dissolved in DMSO800 mMBuffersBuffer SourceKrebs-Ringer Bicarbonate Buffer (KRBG) containing 10 mM Glucose (pH 7.4) Sigma Chemical Company (P/N K4002)Note : 15 mM sodium bicarbonate is not included with this product and must be added separately.Lysis Buffer (pH 7.3) Molecular Devices Corporation (included inCatchPoint cGMP Fluorescent Assay Kit)Phosphate Buffered Saline, PBS Invitrogen Life Technologies(P/N 10010-023)Assay ProtocolAdherent CellsThe following has been used for the adherent cell lines RFL-6 and CHO-K1 :1) Culture cells (1 mL / well) in standard 12-well microtiter plates (tissue culture grade) with cell concentrationat 0.75 – 2.5 x 105 cells/mL.2) Incubate plated cells overnight at 37°C in a humidified atmosphere of 5% CO2 : 95% air.3) Gently aspirate media and slowly add 400 µL of Pre-stimulation Buffer (pH 7.4).Pre-stimulation Buffer (containing 0.75 mM IBMX in KRBG Buffer; make fresh on day of experiment)10 ml of KRBG Buffer (pH 7.4)µL of 800 mM IBMX9.4Note: Once IBMX has been added to the KRBG, mix vigorously to obtain a homogeneous solution.4) Incubate for 10 min at room temperature.5) Add 200 µL of 3X ANP or PBS (negative control). Gently mix and incubate at 37°C for 15 minutes.ANP, 3X (500 nM final concentration)2.0 mL PBS (pH 7.4) containing10 µL of 300 µM ANP (1.5 µM stock)6) Add 200 µL of lysis buffer to each well. Agitate cells to facilitate cell lysis. This can be achieved by shakingthe plate on a plate shaker for 10 min after adding the lysis reagent.7) If desired, carry out microscopic evaluation using Trypan Blue to check if cells have lysed (1:1 ratio). Cellmembrane may still be visible after cell lysis. Lysed cells are now ready for use and should be immediately processed in the cGMP Fluorescent Assay Kit. Use 40 µL per well neat or suitably diluted lysate inadditional lysis buffer (see page 6, Table 5, Step 3 of the Product Insert).8) Proceed with the remainder of the procedure in Table 5.Cells in SuspensionFor non-adherent cells, we recommend centrifugation of the cells from culture medium and suspension of the pellet in Pre-stimulation Buffer (pH 7.4). Add 1 mL (12 well plate) of cell suspension to each well of the plate.It is recommended that you then centrifuge the plates at 1000 rpm for up to 4 min (brake off). Proceed with step3 (shown above) for adherent cells.R3368B。
GP-1670/1670F features• Bright 800 cd/m2 LCD gives excellent readability even in direct sunlight • The LCD and AR glass are bonded together to ensure no fogging issues • Clear visibility even when wearing polarized sunglasses• Internal GPS antenna for simple and easy installation• Standard C-Map 4D chart available on SD card• RotoKey TM revolving menu and familiar point-and-click operation• Internal memory: Waypoint/Track 30,000 points, Route 1,000 routesGP-1670F features• Equipped with FURUNO’s latest digital fish finder technology- Bottom Discrimination Function* * Thru-hull transom transducer mount required.- ACCU-FISH TM — A unique fish size analyzer• Post-processing Gain Control applied to all echoes displayed on the screen • White Line function — Discriminates fish lying near the bottomC-MAP 4DDigital Charts that grow with your boating needs. Dual range chart displayPlotter + Fish Finder* (with ACCU-FISH and Bottom Discrimination function ON) *GP-1670F onlyModelModel5.7" GPS/WAAS CHART PLOTTERSPECIFICATIONS SUBJECT TO CHANGE WITHOUT NOTICEPLEASE READ MANUAL BEFORE USING THE UNIT12093SS Printed in JapanCatalogue No. N-872aAll brand and product names are registered trademarks, trademarks or service marks of their respective holders.GPA-017/017S (Option)Internal GPS Antennaor orScreen Size5.7"Screen Resolution 640 x 480 pixels (VGA)Screen Brightness 800 cd/m 2 (typical)LanguageEnglish (US & UK), French, Spanish, German, Italian, Portuguese, Danish, Swedish, Norwegian, Finnish, Greek, Japanese, Chinese, Russian, Thai, Vietnamese,Polish, Bahase Malaysia, Bahasa Indonesia Display ModesCourse plot, Nav Data, Instruments*, Engine monitor*, Anemometer*, Fuel level gauge*, GPS status,Fish nder** *External sensors required **GP-1670F onlyMemory Capacity30,000 points for ship's track and wayponts 1,000 planned routes (Max. 50 points per route)5,000 quickpoints InterfaceCAN bus: 1 portMini USB*: 1 port (2.0) *for maintenance onlySD card slot: 1 slot (up to 32 GB)ChartC-MAP 4DOther Functions AIS target display* *AIS sensor requiredTemperature -15˚C to +55˚CWaterproo ng IP56Power Supply12-24 VDCConsumption CurrentGP-1670: 0.5 - 0.3 AGP-1670F: 0.9 - 0.5 A (When 600 W transducer used)GPS / WAAS Receive TypeGPS: 50 channels WAAS: 1 channelReceiving Frequency L1 (1575.42 MHz)Accuracy Internal antenna : GPS 10 m Max / MSAS 7 m MaxExternal antenna*: GPS 10 m Max / MSAS 7 m Max*GPA-017 (Option)Time to First FIX 90 s (cold start)Tracking Velocity 999 KnTransmission 600 W or 1 kW* *Matching box MB-1100 required for certain transducers Display Range 5 - 1,200 m, shift: 0 - 1,200 m Extension Mode ACCU-FISH*, Auto (Fishing/Cruising/Manual), A-Scope,Marker Zoom, Bottom Zoom, Bottom Lock, Bottom Discrimination*Picture Advance 7 steps: 2/1, 1/1, 1/2, 1/4, 1/8, 1/16, stop*In some instances, bottom component or sh size indicated on the GP-1670F may differ from its actual size or structure. Please carefully read the operation manual prior to using this feature. Equipment List Display unit GP-1670/1670F, Installation materials and standard spare partsOptions • GPS antenna GPA-017/017S • Antenna cable assembly • NMEA2000 heavy cable assembly • Mast mounting kit • Junction box • In-line Terminator • NMEA2000interface unit • NMEA data converter • Waterproof cap • Termination Resistor• NMEA2000 light cable assembly • Cable assembly • Mini/micro T-connector• L-angle mounting base • Handrail mounting base • Rectifier • T-connector • Inner hull mounting kit* • Matching box* • Speed/Temperature sensor*• Temperature sensor* *for GP-1670F only Transducer Options (for GP-1670F only)600 W 1 kW* *Matching box MB-1100 required for certain transducers • 520-5MSD (Bronze) • 50/200-1T • 50B-6B • 520-5PSD (Plastic) • 50/200-12M • 200B-5S • 525-5PWD (Plastic) • 50B-6ViewableConventional GP-1670Easy Replacement from legacy modelsNMEA DATA CONVERTER < IF-NMEA2K2 >IF-NMEA2K2Furuno’s NMEA DATA CONVERTER is a compact interface unit, that converts NMEA 0183 data into CAN bus/NMEA2000 data and vice-versa. Control data such as Heading and Rate-of Turn can be translated in high speed conversion rate.Black out。